Simultaneous determination of six antibiotics belonging to four different classes in chicken meat BY HPLC/DAD and verification BY LC-MS/MS

DOYUK F., DOST K.

Food Chemistry, cilt.426, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 426
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.foodchem.2023.136549
  • Dergi Adı: Food Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Chicken meat, Tetracyclines, Chloramphenicol, Enrofloxacin, Penicillin G, HPLC-DAD
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

The hypothesis of this study was to develop an extraction method allowing side-by-side extraction of six antibiotics belonging to four different classes and an HPLC/DAD method for the determination of their residues in chicken breast meat. The validation data showed that this hypothesis was achieved. The results were then confirmed by LC-MS/MS method. Sample preparation was based on the classical solid–liquid extraction with methanolic citric acid. The average recoveries were satisfactory and ranged from 75.68 to 101.3%. The linearity of the developed HPLC/DAD method was very high in the concentration range studied (R2 > 0.9969). The accuracy and precision of the analytical method were between –10.8 and 12.0% relative error and 0.82 to 10.1% relative standard deviation, respectively. The LODs for five antibiotics ranged from 0.6 to 2.7 µg kg−1 and LOQs ranged from 2.0 to 8.9 µg kg−1. For penicillin G, the LOD was 0.16 and LOQ was 0.52 mg kg−1.