Determination of the phytoalexin resveratrol (3,5,4′-Trihydroxystilbene) in peanuts and pistachios by High-Performance Liquid Chromatographic Diode Array (HPLC-DAD) and Gas Chromatography-Mass Spectrometry (GC-MS)

TOKUŞOĞLU Ö., Ünal M. K., Yemiş F.

Journal of Agricultural and Food Chemistry, cilt.53, sa.12, ss.5003-5009, 2005 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 12
  • Basım Tarihi: 2005
  • Dergi Adı: Journal of Agricultural and Food Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.5003-5009
  • Anahtar Kelimeler: resveratrol, trans-resveratrol, cis-resveratrol, peanut, pistachio, HPLC-DAD, GC-MS
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

The phytoalexin resveratrol (3,5,4′-trihydroxystilbene) in edible peanut (Arachis hypogaea L.) and pistachio (Pistacia vera L.) varieties grown in Turkey was analyzed by high-performance liquid chromatographic diode array and gas chromatography-mass spectrometric detection. trans-Resveratrol in six peanut varieties, five pistachio varieties, and four market samples ranged between 0.03 and 1.92 μg/g. The Çerezlik 5025 peanut (1.92 ± 0.01 μg/g) and Ohadi pistachio genotype (1.67 ± 0.01 μg/g) had significantly higher trans-resveratrol contents. Peanuts contained 0.03-1.92 μg/g (av = 0.84 μg/g) of trans-resveratrol, whereas pistachio contained 0.09-1.67 μg/g (av = 1.15 μg/g). With exposure to UV light for 1 min, trans-resveratrol concentrations of samples ranged from 0.02 to 1.47 μg/g and those of cis-resveratrol from 0.008 to 0.32 μg/g. The occurrence of resveratrol in peanut and pistachio was confirmed by total ion chromatograms (TIC) of bis[trimethylsilyl]-trifluoroacetamide derivatives of resveratrol isomers and comparison of the mass spectral fragmentation data with those of a resveratrol standard. Formation of the cis-isomer in pistachios was higher than in peanuts. © 2005 American Chemical Society.