Fatty acid and conjugated linoleic acid profiles of infant formulas through direct transesterification of acyl lipids

TOKUŞOĞLU Ö., Durucasu I., Akalin A., Serin E., Akşit S.

Italian Journal of Food Science, vol.19, no.4, pp.477-484, 2007 (SCI-Expanded, Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 4
  • Publication Date: 2007
  • Journal Name: Italian Journal of Food Science
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.477-484
  • Keywords: Conjugated linoleic acids, Fatty acids, Infant formulas
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

Fatty acid (FA) values, including C8:0, C10:0, C12:0, C13:0, C14:0, C16:0, C18:0, C18:l t, C18:l c, C18:2, C18:2 CLA, C20:0, C18:3 and C22:0, in sixteen infant formulas were determined through direct transesterification of acyl lipids with sodium methoxide by capillary gas chromatography. Total FA values ranged from 250.25-256.06 mg/g sample and the conjugated linoleic acid (CLA) (cis-9, trans-11 octadecadienoic acid) values were 1.41-2.02 mg/g sample (p<0.01) in the infant formulas. The total saturated fatty acid, monounsaturated fatty acid and polyunsaturated fatty acid values were also obtained. The C18:3 (linolenic acid (n-3)) values were 0.63-0.88 mg/g sample, whereas the C18:2 (linoleic acid (n-6)) values ranged from 35.64-35.82 mg/g sample. FA standard mix including CLA had linear calibration curves through the origin (R2 = 0.9999). The precision of the analytical method was (using C13:0, methyl tridecanoate, as internal standard) within the 95% confidence limits and the mean recoveries determined for individual fatty acids in infant formulas varied from 99.8 to 100%.