Evaluation of non-invasive clinical samples in chronic Chlamydial prostatitis by using In situ hybridization


Gümüş B., Sengil A. Z., Solak M., Fistik T., Alibey E., Çakmak E. A., ...More

Scandinavian Journal of Urology and Nephrology, vol.31, no.5, pp.449-451, 1997 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 5
  • Publication Date: 1997
  • Doi Number: 10.3109/00365599709030640
  • Journal Name: Scandinavian Journal of Urology and Nephrology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.449-451
  • Keywords: chronic non-bacterial prostatitis, C-trachomatis, in situ hybridization
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

Seventy-eight non-invasive prostate specimens collected from patients with chronic non-bacterial prostatitis were evaluated by in situ hybridization (IH) for evidence of Chlamydia trachomatis. Intracellular Chlamydia bodies were detected in 18 of 78 cases (20.6%). Homogeneous blue- black bodies in the cellular cytoplasm were accepted as in situ positive. Chlamydial antigen detected by enzyme immuno assay (EIA) was positive in 12 cases (13.7%), but only nine of them were positive by IH. Our study confirms previous reports implicating C. trachomatis as an aetiological agent in chronic non-bacterial prostatitis, and underscores the applicability of DNA probes for detection and identification of C. trachomatis in prostatic materials.