Investigation of effect of Vitamin D receptor, calcium-sensing receptor and β-catenin on cutaneous squamous cell carcinoma D vitamini reseptörü, kalsiyuma duyarlı reseptör ve β-katenin'in skuamöz hücreli deri kanseri üzerine etkisinin araştırılması

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TUĞRUL B., Söylev S., TEMİZ P., Gençoğlan G.

Turkish Journal of Biochemistry, vol.45, no.1, pp.91-98, 2020 (SCI-Expanded, Scopus, TRDizin) identifier

  • Publication Type: Article / Article
  • Volume: 45 Issue: 1
  • Publication Date: 2020
  • Doi Number: 10.1515/tjb-2019-0434
  • Journal Name: Turkish Journal of Biochemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, EMBASE, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.91-98
  • Keywords: CaSR, Cutaneous squamous cell carcinoma, Immunohistochemistry, VDR, Western blotting, β-catenin
  • Open Archive Collection: AVESIS Open Access Collection
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

Background: Cutaneous squamous cell carcinoma (cSCC) is a malignant and invasive tumor which is originated from epidermis with a high incidence among non-melanoma skin cancers. The aim of this study was to determine whether vitamin D receptor (VDR), calcium-sensing receptor (CaSR) and beta catenin (β-catenin) proteins have an effect on cSCC. Materials and methods: VDR, CaSR and β-catenin proteins in tissue samples of cSCC and control group were analyzed by immunohistochemistry (IHC) and Western blotting (WB) method. IHC findings were statistically evaluated. Results: IHC staining density of VDR and β-catenin were higher in cSCC tissue samples than control. The difference between IHC staining density of VDR and β-catenin in the patient and the control groups were statistically significant (p = 0.021, p = 0.021, respectively), but not for CaSR (p = 0.237). While the VDR and β-catenin staining rates obtained by the IHC method could be supported by WB results, the WB bands for CaSR could not be shown. Conclusion: The findings suggest that VDR and β-catenin may have an effect on the disease. Further research is required to better understand the role of VDR and β-catenin together on cSCC.