Investıgatıon of the adherence and prolıferatıon characterıstıcs of SH-SY5Y neuron model cells on graphene foam surfaces

Morçimen Z. G., Taşdemir Ş., Erdem Ç., Güneş F., Şendemir A.

2018 International Conferences and Exhibition on Nanotechnologies - Organic Electronics and Nanomedicine, Nanotexnology 2018, Thessaloniki, Greece, 30 June - 06 July 2018, vol.19, pp.40-46, (Full Text) identifier

  • Publication Type: Conference Paper / Full Text
  • Volume: 19
  • Doi Number: 10.1016/j.matpr.2019.07.654
  • City: Thessaloniki
  • Country: Greece
  • Page Numbers: pp.40-46
  • Keywords: Biomaterials, Foam, Graphene, Neural tissue engineering, Scaffolds, SH-SY5Y
  • Manisa Celal Bayar University Affiliated: No

Abstract

Graphene is a promising material for production of biocompatible surfaces with suitable physicochemical properties for effective adhesion, proliferation and differentiation of nerve cells. In this study, 3-dimensional (3D) graphene foam was synthesized via chemical vapour deposition method, and it was aimed to observe the effects of graphene foam scaffolds on SH-SY5Y dopaminergic neuron model cells before applying to clinics. Raman spectroscopy, XRD and SEM analyses were performed to characterize the graphene foam. Viability of SH-SY5Y cells cultured on graphene foam scaffolds was analysed on days 1, 4 and 7 by MTT. Furthermore, cell morphology was determined by fluorescence staining of the actin cytoskeleton.