Investıgatıon of the adherence and prolıferatıon characterıstıcs of SH-SY5Y neuron model cells on graphene foam surfaces

Morçimen Z. G., Taşdemir Ş., Erdem Ç., Güneş F., Şendemir A.

2018 International Conferences and Exhibition on Nanotechnologies - Organic Electronics and Nanomedicine, Nanotexnology 2018, Thessaloniki, Yunanistan, 30 Haziran - 06 Temmuz 2018, cilt.19, ss.40-46, (Tam Metin Bildiri) identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası: 19
  • Doi Numarası: 10.1016/j.matpr.2019.07.654
  • Basıldığı Şehir: Thessaloniki
  • Basıldığı Ülke: Yunanistan
  • Sayfa Sayıları: ss.40-46
  • Anahtar Kelimeler: Biomaterials, Foam, Graphene, Neural tissue engineering, Scaffolds, SH-SY5Y
  • Manisa Celal Bayar Üniversitesi Adresli: Hayır

Özet

Graphene is a promising material for production of biocompatible surfaces with suitable physicochemical properties for effective adhesion, proliferation and differentiation of nerve cells. In this study, 3-dimensional (3D) graphene foam was synthesized via chemical vapour deposition method, and it was aimed to observe the effects of graphene foam scaffolds on SH-SY5Y dopaminergic neuron model cells before applying to clinics. Raman spectroscopy, XRD and SEM analyses were performed to characterize the graphene foam. Viability of SH-SY5Y cells cultured on graphene foam scaffolds was analysed on days 1, 4 and 7 by MTT. Furthermore, cell morphology was determined by fluorescence staining of the actin cytoskeleton.