Immobilization of catalase on chitosan and amino acid-modified chitosan beads

Başak E., AYDEMİR T.

Artificial Cells, Nanomedicine and Biotechnology, cilt.41, sa.4, ss.269-275, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 4
  • Basım Tarihi: 2013
  • Doi Numarası: 10.3109/10731199.2012.742095
  • Dergi Adı: Artificial Cells, Nanomedicine and Biotechnology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.269-275
  • Anahtar Kelimeler: Amino acid, catalase, chitosan, immobilization, modification
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

Bovine liver catalase was covalently immobilized onto amino acid-modified chitosan beads. The beads were characterized with SEM, FTIR, TGA and the effects of immobilization on optimum pH and temperature, thermostability, reusability were evaluated. Immobilized catalase showed the maximal enzyme activity at pH 7.0 at 30°C. The kinetic parameters, Km and Vmax, for immobilized catalase on alanine-chitosan beads and lysine-chitosan beads were estimated to be 25.67 mM, 27 mM and 201.39 μmol H2O 2/min, 197.50 μmol H2O2/min, respectively. The activity of the immobilized catalase on Ala-CB and Lys-CB retained 40% of its high initial activity after 100 times of reuse. © 2013 Informa Healthcare USA, Inc.