Investigation of therapeutic efficiency of phenytoin (PHT) labeled with radioactive 131I in the cancer cell lines

Uzaras C., AVCIBAŞI U., Demiroğlu H., Medine E. İ., Kılçar A. Y., Müftüler F. Z. B., ...More

Journal of Radioanalytical and Nuclear Chemistry, vol.307, no.1, pp.131-140, 2016 (SCI-Expanded, Scopus) identifier

  • Publication Type: Article / Article
  • Volume: 307 Issue: 1
  • Publication Date: 2016
  • Doi Number: 10.1007/s10967-015-4119-0
  • Journal Name: Journal of Radioanalytical and Nuclear Chemistry
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.131-140
  • Keywords: Daoy and A549 cancerous cell lines, Diphenylhydantoin, Epilepsy, Phenytoin, Radiolabeling, U-87 MG
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

The aim of this study is to determine the incorporations of PHT radiolabeled with 131I (131I-PHT) on U-87 MG, Daoy and A549 cancerous cell lines. For this, cold and radio-labeling studies were carried out. The radio-labeling yield of 131I-PHT was obtained about 95 %. Subsequently, cell culture studies were carried out and radio-labeling yields of 131I, 131I-PHT on U-87 MG, Daoy and A549 cancerous cells were investigated. Cell culture studies demonstrated that the incorporation values of 131I-PHT on the three cell lines decreased with increasing radioactivity. Consequently, 131I-PHT may be a good radiopharmaceutical for targeting radionuclide therapy of Central Nervous System Tumors.