Akademik Veri Yönetim Sistemi
South African Journal of Botany, cilt.184, ss.761-768, 2025 (SCI-Expanded, Scopus)
Immiscibility of oils in aqueous solution interferes with the in vitro antibacterial/anticancer activity testing and content analyses of essential oils. In the present work, we reported a unique technique using DMSO as an extractant to determine in vitro biological activities and total phenolic constituents of the clove oil (D-co). Total phenolic contents, total flavonoid contents and antioxidant capacity of D-co extract were determined using Folin-Ciocalteu, AlCl3 and CUPRAC colorimetric assays, respectively. LC-MS/MS and GC–MS analyses were also performed to reveal the phytochemicals in the obtained extract. To test the biological activities of D-co extract, broth dilution method for antibacterial activity, MTT cell proliferation assay for in vitro anticancer activity, and microscope analysis for cell morphology were performed. According to our findings, D-co extract displayed a significant total phenolic content, total flavonoid content, and antioxidant capacity with 1274.87±11.65 µg GAE/g D-co, 950.60±36.67 µg CE/g D-co and 256.28±2.59 mM Trolox equivalent, respectively. Among 32 compounds analyzed in LC-MS/MS, vanillin was found to be the most dominant compound, with 1070.72±9.05 mg/L. GC–MS analysis revealed that isoaromadendrene epoxide, eugenol acetate, caryophyllene, and vanillin are the major compounds of D-co extract. D-co showed in vitro antibacterial and anticancer activities. The IC50 value of D-co was determined to be 5.5 mg/mL for human bladder cancer (T24) cells at 48 h. Upon D-co exposure, significant morphological alterations were observed on T24 cells under a light microscope. In conclusion, a fast and facile method using DMSO as an extractant was presented for the first time to analyze the phytochemical components and biological activities of an essential oil.