Research Information System
Cyprus Journal of Medical Sciences, vol.10, no.1, pp.65-68, 2025 (ESCI, TRDizin)
BACKGROUND/AIMS: The goal of this research was to analyze the cytotoxic impacts of rosemary essential oil on pancreatic cancer. MATERIALS AND METHODS: The human pancreatic cancer cell line (PANC-1) was used. The leaves of rosemary cultivated in North Cyprus were collected during the flowering stage. Rosemary essential oil was obtained after air-dried leaves (100 g) were distilled with water for 3 hours. Rosemary essential oil solutions were prepared and diluted in culture medium using various concentrations (100, 200, 300, 400, 500, and 600 μM) for 24 hours and 48 hours incubation periods. The analysis of cytotoxicity was conducted using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide]. RESULTS: Rosemary oil at 500 µM was more effective in diminishing the proliferation of PANC-1 cells than other concentrations during 48 hours. Cell viability was significantly greater at 100 μM for 24 hours (100%) than 600 μM for 48 hours (81.48%). It was determined that substantially greater cell viability was observed at 200 μM for 24 hours (97.05%) when compared to 600 μM for 48 hours (81.48%). In addition, when various concentrations were evaluated during 48 hours, significant differentiation was found between 100 μM and 600 μM. It was shown that there was a significant differentiation between 200 μM and 600 μM for 48 hours. CONCLUSION: Cell viability was substantially less at 600 μM than at 100 μM and 200 μM, 81.48%, 95.18%, and 90.23%, respectively.