Akademik Veri Yönetim Sistemi
Cyprus Journal of Medical Sciences, cilt.10, sa.1, ss.92-95, 2025 (ESCI, TRDizin)
BACKGROUND/AIMS: Stem cells that have the ability to differentiate into other somatic cells are special cells in tissue and play a role in the repair and regeneration of tissues. Menstrual blood stem cells (MenSCs), a subtype of mesenchymal stem cells derived from the endometrium during menstruation, present an accessible and non-invasive source for stem cell research. This study aimed to create a protocol to obtain MenSCs from menstrual blood. MATERIALS AND METHODS: Samples were collected from fertile women aged 18-45 on the second day of their menstrual cycles. Two methods: Ficoll and collagenase, were applied for cell isolation. In the Ficoll method, solution was slowly added in equal proportions and then centrifuged at 2000 rpm for twenty minutes (min). MenSCs were collected, centrifuged at 1000 rpm for 10 min, and then cultured in MenSCs culture medium at 37 °C with 5% CO2 in air. In the collagenase technique, 0.5 mg/mL collagenase 1 was added to the samples in a 1:1 ratio and left to incubate for 1 hour at 37 °C with 5% CO2 in air. After centrifugation, MenSCs were cultured and were then passaged three times. Distributions of CD31 and CD44 were used to characterize the cells via the indirect immunoperoxidase technique. RESULTS: Cells isolated using both methods exhibited epithelioid morphology, cytoplasmic lipid droplets and colony formation. However, cells obtained with the collagenase method demonstrated faster proliferation and formed bigger colonies. Morphology and immunoreactivity remained consistent across passages. CONCLUSION: MenSCs isolated with collagenase method showed better proliferation than those obtained with ficoll. The immunocytochemistry results, showing CD31 negativity and CD44 positivity, confirmed the mesenchymal stem cell characteristics of the isolated cells.