Akademik Veri Yönetim Sistemi
Cyprus Journal of Medical Sciences, cilt.10, sa.1, ss.87-91, 2025 (ESCI, TRDizin)
BACKGROUND/AIMS: Mesenchymal stem cells (MSCs) are considered potential candidates in regenerative medicine due to their angiogenic, anti-apoptotic, and immunomodulatory properties. Among the secretory products of MSCs are biomolecules such as cytokines, chemokines, growth factors, and particularly exosomes. Exosomes are nanovesicles, ranging from 20 to 100 nm, that can be isolated from various body fluids. RAB31 plays a role in an endosomal sorting complex required for transport-independent pathway. Our study aims to determine the changes in exosome biogenesis and secretion of RAB31 from adipogenic mesenchymal stem cells (AMSCs), cultured in 2D and 3D culture conditions under normoxic and hypoxic environments. MATERIALS AND METHODS: AMSCs were cultured in 2D and 3D conditions, and the study groups were formed as follows: normoxic 2D AMSC culture (group 1), normoxic 3D AMSC culture (group 2), hypoxic 2D AMSC culture (group 3), and hypoxic 3D AMSC culture (group 4). After culturing all groups of cells under normoxic and hypoxic conditions for 48 hours, exosomes were collected from culture medium, and the presence of RAB31, Rab7, CD9, and CD63 proteins was evaluated using indirect immunocytochemistry. RESULTS: The exosome secretion was different after normoxic and hypoxic conditions. In addition, the distributions of RAB31 and RAB7 were different, but the intensity of CD9 and CD63 immunoreactivity was similar in 2D conditionions. CD9 immunoreactivity was not affected under hypoxic or normoxic conditions. However, increased immunoreactivity of CD63 was observed. Moderate and negative RAB7 immunoreactivity was observed in 3D hypoxic and normoxic conditions, respectively. RAB31 immunoreactivity was higher in hypoxic conditions than in normoxic conditions. CONCLUSION: Exosome secretion from AMSCs was affected after culture conditions. Especially hypoxic conditions triggers the secretion of RAB31, and co-localization of CD63 and RAB31 under these conditions points out that exosome biogenesis controls RAB31 in AMSCs under hypoxic conditions.