Research Information System
Waste and Biomass Valorization, 2025 (SCI-Expanded, Scopus)
Purpose: This study aims to evaluate the bioactive potential of dry coffee parchment powder (DCPP) by investigating its phytochemical composition specifically polyphenols, flavonoids, and condensed tannins as well as its in vitro biological properties and in vivo acute toxicity effects. Methods: The research involved phytochemical quantification of aqueous coffee parchment extract (ACPE) to determine polyphenol, flavonoid, and condensed tannin content. The biological properties were assessed through antioxidant activity and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to evaluate cell viability at different ACPE concentrations (375–1500 µg/ml). Additionally, in vivo acute toxicity tests were conducted on male and female rats at varying DCPP doses (25%, 50%, 75%, and 85%). Results: The ACPE was found to be rich in polyphenols (105.793 ± 4.230 mg GAE/g DW), flavonoids (13.147 ± 4.169 mg CE/g DW), and condensed tannins (82.07 ± 3.942 mg CE/g DW). The extract exhibited more than moderate antioxidant potency. The MTT assay revealed that cell viability remained above 50% at lower ACPE concentrations. Acute toxicity tests indicated that high doses of DCPP (75% and 85%) led to physiological changes, with females being more affected, highlighting a sex-based difference in response. Conclusion: Dry coffee parchment powder presents promising bioactive properties and can be used as a natural additive in controlled doses across various applications, including livestock feed, cosmetics, and pharmaceuticals.