Comperative study of catalase immobilization on chitosan, magnetic chitosan and chitosan-clay composite beads

Başak E., AYDEMİR T., DİNÇER A., ÇINAR S.

Artificial Cells, Nanomedicine and Biotechnology, vol.41, no.6, pp.408-413, 2013 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 41 Issue: 6
  • Publication Date: 2013
  • Doi Number: 10.3109/10731199.2013.796312
  • Journal Name: Artificial Cells, Nanomedicine and Biotechnology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.408-413
  • Keywords: catalase, composite beads, comparative study, immobilization, reusability
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

Catalase was immobilized on chitosan and modified chitosan. Studies were carried out on free-immobilized catalase concerning the determination of optimum temperature, pH, thermal, storage stability, reusability, and kinetic parameters. Optimum temperature and pH for free catalase and catalase immobilized were found as 35°C and 7.0, respectively. After 100 times of repeated tests, the immobilized catalases on chitosan-clay and magnetic chitosan maintain over 50% and 60% of the original activity, respectively. The ease of catalase immobilization on low-cost matrices and good stability upon immobilization in the present study make it a suitable product for further use in the food industry. © 2013 Informa Healthcare USA, Inc.