Comperative study of catalase immobilization on chitosan, magnetic chitosan and chitosan-clay composite beads

Başak E., AYDEMİR T., DİNÇER A., ÇINAR S.

Artificial Cells, Nanomedicine and Biotechnology, cilt.41, sa.6, ss.408-413, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 6
  • Basım Tarihi: 2013
  • Doi Numarası: 10.3109/10731199.2013.796312
  • Dergi Adı: Artificial Cells, Nanomedicine and Biotechnology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.408-413
  • Anahtar Kelimeler: catalase, composite beads, comparative study, immobilization, reusability
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

Catalase was immobilized on chitosan and modified chitosan. Studies were carried out on free-immobilized catalase concerning the determination of optimum temperature, pH, thermal, storage stability, reusability, and kinetic parameters. Optimum temperature and pH for free catalase and catalase immobilized were found as 35°C and 7.0, respectively. After 100 times of repeated tests, the immobilized catalases on chitosan-clay and magnetic chitosan maintain over 50% and 60% of the original activity, respectively. The ease of catalase immobilization on low-cost matrices and good stability upon immobilization in the present study make it a suitable product for further use in the food industry. © 2013 Informa Healthcare USA, Inc.