Comparative analysis of XTT assay and xCELLigence system by measuring cytotoxicity of resveratrol in human cancer cell lines İnsan kanser hücre hatlarında resveratrol sitotoksisitesinin XTT testi ve xCELLigence sistemi ile karşılaştırmalı analizi

ATMACA İLHAN H., Bozkurt E., Kısım A., Uslu R.

Turkish Journal of Biochemistry, cilt.41, sa.6, ss.413-421, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 41 Sayı: 6
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1515/tjb-2016-0128
  • Dergi Adı: Turkish Journal of Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.413-421
  • Anahtar Kelimeler: Resveratrol, Cytotoxicity, XTT, xCELLigence, Human cancer cell lines
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

Objective: In vitro preliminary oncological and translational studies are mainly based on evaluating the cytotoxic effects of a specific compound on cultured cells. Resveratrol is a commercially available compound which is originally isolated from the roots of white hellebore and later from Polygonum cuspidatum. The objective of the study was to compare cytotoxicity data of Resveratrol from XTT end point assay with a real-time cell based xCELLigence system in terms of accuracy, sensitivity, speed and reproducibility in a panel of human cancer cell lines. Methods: XTT end point assay and real-time cell based xCELLigence system were used to evaluate cytotoxicity. Cytotoxicity results were verified by monitoring cells under phase-contrast microscope which were treated with IC50 values of resveratrol. Results: Resveratrol decreased cell viability in a timeand concentration-dependent manner in all cancer cell lines when tested by both the XTT assay and xCELLigence system. Standard deviations of the xCELLigence data were found to be lower than the data from XTT assay. Conclusion: The data from this study strongly imply that xCELLigence system has higher precision, more enlightening and more reproducible than XTT end point assay.