Contractility of diabetic human corpus cavernosum smooth muscle in response to serotonin mediated via Rho-kinase

DURMUŞ SÜTPİDELER N., Soner B. C., Demir O., ESEN A. A., GİDENER S.

Pharmacology, cilt.84, sa.1, ss.24-28, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 84 Sayı: 1
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1159/000221380
  • Dergi Adı: Pharmacology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.24-28
  • Anahtar Kelimeler: Corpus cavernosum, human, Diabetes mellitus, Rho-kinase, Serotonin
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

Background/Aims: Serotonin (5-hydroxytryptamine, 5-HT)-induced contraction and the involvement of RhoA/Rho-kinase pathway in the 5-HT-induced contraction was investigated isometrically in vitro in both diabetic and nondiabetic human corpus cavernosum (HCC) tissues. Methods: HCC tissues were obtained from 12 patients. The response to 5-HT (10-9 to 10-5 mol/l) was studied in isolated HCC tissues in the absence and in the presence of a Rho-kinase inhibitor (Y-27632). Results: Preincubation with Y-27632 attenuated maximum contractions induced by 5-HT in tissues of both nondiabetics and diabetics. When diabetic and nondiabetic groups were compared, no significant difference was seen in 5-HT-induced contraction alone, but in the presence of Y-27632, 5-HT-induced contraction was significantly higher in the diabetic group. Conclusion: These results suggest that the Rho-kinase-mediated pathway plays an important role for 5-HT-induced contraction in diabetic corpus cavernosum tissues. Copyright © 2009 S. Karger AG.