Association of platelet-activating factor acetylhydrolase gene polymorphism with premature coronary artery disease in Turkish patients

Şekuri C., ÇAM F. S., Tengiz I., Ercan E., BAYTURAN Ö., Berdeli A.

Anadolu Kardiyoloji Dergisi, vol.6, no.2, pp.132-134, 2006 (Scopus, TRDizin) identifier identifier

  • Publication Type: Article / Article
  • Volume: 6 Issue: 2
  • Publication Date: 2006
  • Journal Name: Anadolu Kardiyoloji Dergisi
  • Journal Indexes: Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.132-134
  • Keywords: Gene, PAF acetylhydrolase, Polymorphism, Premature coronary artery disease
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

Objective: Platelet-activating factor (PAF) is a phospholipid with multiple actions that is involved in inflammatory diseases as well as in atherogenesis. It is inactivated by a plasma enzyme, PAF-acetylhydrolase (PAF-AH). Deficiency of this enzyme in plasma is caused by a missense mutation in the gene (G994T). The aim of this study was to investigate association of this mutation with premature coronary artery disease (CAD). Methods: One hundred and fifteen unrelated Turkish patients with a diagnosis of premature CAD and 128 unrelated healthy subjects were enrolled in this study. Genotyping was performed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP). Results: The prevalence of the G994T mutation in the patients was 2.60 % (heterozygote), and 0 % in the controls. There was no significant difference in allele frequency and genotype distribution among the study groups. Conclusion: The G9943T mutation in the plasma PAF acetylhydrola se gene is not associated with premature CAD in Turkish subjects.