Caffeic Acid Detection Using an Inhibition-Based Lipoxygenase Sensor

ODACI DEMİRKOL D., Gulsunoglu B., Ozdemir C., DİNÇER A., ZİHNİOĞLU F., TİMUR S.

FOOD ANALYTICAL METHODS, sa.2, ss.244-249, 2012 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s12161-011-9226-0
  • Dergi Adı: FOOD ANALYTICAL METHODS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.244-249
  • Anahtar Kelimeler: Biosensor, Lipoxygenase, Caffeic acid, Carbon paste electrode
  • Manisa Celal Bayar Üniversitesi Adresli: Hayır

Özet

An inhibition based biosensing system was developed for the caffeic acid as lipoxygenase (LOX) inhibitor. LOX was immobilized in carbon paste electrode and the amperometric detection of hydroperoxy linoleic acid due to the enzymatic reaction using linoleic acid as a substrate was monitored at +0.9 V versus Ag/AgCl. The decrease in biosensor response in the presence of caffeic acid was found to be correlated with the inhibitor concentration. Diode array detector and LOX biosensor was used as an electrochemical detector for the analysis of this compound. All data were given as a comparison of two systems.