Portable quantification of silver ion by using personal glucose meter (PGM) and magnetite cross-linked invertase aggregates (MCLIA)

POLATOĞLU İ., Yardım A.

Analytical Biochemistry, cilt.643, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 643
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.ab.2021.114527
  • Dergi Adı: Analytical Biochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Compendex, EMBASE, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: PGM, Ag+ detection, Portable quantification, Enzyme aggregates, Invertase inhibition
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

Heavy metal detection is critical due to its harmful effects on human health and the ecosystem. Enzyme-based platforms attract attention for heavy metal detection such as silver, a toxic metal, due to being small, portable, and requiring only essential equipment compared with the basic analytical methods. In this study, magnetic cross-linked invertase aggregates (MCLIA) were developed for the first time as an enzyme-based signaling platform to detect Ag+ using a personal glucose meter (PGM). EDX, FTIR, and VSM results depicted that MCLIA was successfully developed and exhibits super-paramagnetism. In addition, MCLIA selectively detected the Ag+ at a sensitivity of 1.2 inhibition rate/μM in a linear range from 5 to 70 μM with a detection limit of 4.6 μM and IC50 value of 42.3 μM. These findings strongly indicate that MCLIA is applicable as a signal platform for portable quantification of other analytes that inhibits the invertase enzyme.