MYC-driven regulation of long non-coding RNA profiles in breast cancer cells

Tokgun P. E., Tokgun O., KURT S., Tomatir A. G., Akca H.

Gene, vol.714, 2019 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 714
  • Publication Date: 2019
  • Doi Number: 10.1016/j.gene.2019.143955
  • Journal Name: Gene
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

Aim: MYC deregulation contributes to breast cancer development and progression. Deregulated expression levels of long non-coding RNAs (lncRNA) have been demonstrated to be critical players in development and/or maintenance of breast cancer. In this study we aimed to evaluate lncRNA expressions depending on MYC overexpression and knockdown in breast cancer cells. Materials and methods: Cells were infected with lentiviral vectors by either knockdown or overexpression of c-MYC. LncRNA cDNA was transcribed from total RNA samples and lncRNAs were evaluated by qRT-PCR. Results: Our results indicated that some of the lncRNAs having tumor suppressor (GAS5, MEG3, lincRNA-p21) and oncogenic roles (HOTAIR) are regulated by c-MYC. Conclusion: We observed that c-MYC regulates lncRNAs that have important roles on proliferation, cell cycle and etc. Further studies will give us a light to identify molecular mechanisms related to MYC-lncRNA regulatory pathways in breast cancer.