The Effect of N-Acetylcysteine on the Neurofilament and Nerve Growth Factor Expression after Gentamicin Induced Cochlear Damage Gentamisin ile Oluşturulan Kohlear Hasar üzerine N-Asetilsisteinin Nörofilament ve Sinir Büyüme Faktörü Salınımına Etkisi

Sarsmaz H. Y., GÜRGEN S. G., Somdaş M. A.

Gazi Medical Journal, cilt.33, sa.3, ss.232-237, 2022 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.12996/gmj.2022.54
  • Dergi Adı: Gazi Medical Journal
  • Derginin Tarandığı İndeksler: Scopus, Academic Search Premier
  • Sayfa Sayıları: ss.232-237
  • Anahtar Kelimeler: Gentamicin, Drug Induced Ototoxicity, N-acetylcysteine, Cochlea, Nerve growth factor, Neurofilament protein
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

Objective: To investigate of the effectiveness of N-acetylcysteine (NAC) in preventing cochlear damage due to gentamicin by the means of expression of neuronal factors Metods: In our study, 36 female Spraguea Dawley rats were used. They were divided into 3 groups such as (saline), Gentamicin + Serum physiological (saline) and Gentamicin + NAC. After 15 days, the rats were sacrificed. Cochleae were removed and examined histopathologically. The immunoreactivity of neurofilament and neuronal growth factor primary antibodies was examined in the tissues taken. Results: Neuroflament immunoreactivity was quite significant in afferent and efferent nerve bundles in the saline group and Gentamicin + NAC group, whereas it was weak in the Gentamicin + serum physiological group. Also neuronal growth factor immunoreactivity was moderate in afferent and efferent nerve bundles in the saline group and Gentamicin + NAC group, it was negative at vestibular ganglia, and weak in afferent and efferent nerve fiber bundles in the Gentamicin + serum physiological group. Conclusion: It was shown immunohistochemically that ototoxicity caused by gentamicin was significantly reduced when the NAC combination was applied.