Long-term storage of microalgae: determination of optimum cryopreservation conditions

DENİZ CAN İ., Demirel Z., İMAMOĞLU E., Conk-Dalay M.

Journal of the Marine Biological Association of the United Kingdom, vol.102, no.3-4, pp.276-284, 2022 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 102 Issue: 3-4
  • Publication Date: 2022
  • Doi Number: 10.1017/s0025315422000479
  • Journal Name: Journal of the Marine Biological Association of the United Kingdom
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Geobase, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.276-284
  • Keywords: Chlorella vulgaris, cryopreservation, microalgae, Neochloris texensis, optimization
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

Maintenance of eukaryotic microalgae strains for the long term is generally carried out using serial subculture techniques which require labour, time and cost. Cryopreservation techniques provide long-term storage of up to years for numerous microorganism strains and cell cultures. Ssu930ijn vbvbhnn8;l,n is related to a successfully designed mass and heat transfer balance throughout the cell. In this study, optimization of the cryopreservation process was carried out for two commercially used microalgal strains. The parameters to be optimized were DMSO percentage (0-25%), incubation time (1-15 min) and cryopreservation term (7-180 days) using a central composite design (CCD). Long-term storage up to 123.17 and 111.44 days corresponding to high cell viabilities was achieved for Chlorella vulgaris and Neochloris texensis, respectively. Generated models were found to be in good agreement with experimental results. The study also revealed holistic results for storage of microalgal strains in a stable state for industrial applications.