Differentiation of Entamoeba histolytica and Entamoeba dispar by PCR: A preliminary study in Izmir, Turkey

Dagci H., Erdogan D. D., TÖZ S., Kurt O., Ustun S., Akarca U.

New Microbiologica, vol.30, no.1, pp.45-48, 2007 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Review
  • Volume: 30 Issue: 1
  • Publication Date: 2007
  • Journal Name: New Microbiologica
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.45-48
  • Keywords: Differentiation, Entamoeba dispar, Entamoeba histolytica
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

The causative agent of amoebiasis is currently attributed to two distinct species (E.histolytica and E.dispar). The aim of this study was to differentiate these species by PCR in stool samples. Isolated genomic DNA was amplified by PCR and band products of 101 bp (E. dispar) were obtained. All seven stool samples were found to be E. dispar, not E.histolytica. Our results demonstrated the significance of E.histolytica/dispar differentiation in the diagnosis of amoebiasis. This study is preliminary to our current research project entitled "Investigation of the prevalence of amoebiasis and Entamoeba species in Izmir and its hinterland".