Preparation of a 99mTc-labeled graft polymer and its in vitro and in vivo evaluation

AVCIBAŞI U., Türkyarar T., Karadağ A., Bakan B., Yavaşoğlu N. Ü. K., Kuşat K., ...Daha Fazla

Journal of Radioanalytical and Nuclear Chemistry, cilt.329, sa.2, ss.511-525, 2021 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 329 Sayı: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s10967-021-07817-6
  • Dergi Adı: Journal of Radioanalytical and Nuclear Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, INSPEC, Metadex, Pollution Abstracts, Public Affairs Index, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.511-525
  • Anahtar Kelimeler: 99mTc, Cytotoxicity, Graft Polymer, Nanopolymer, Proline, Radiolabeling
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

The aim of this study is the synthesis of a novel 99mTc-labeld graft polymer and the biological evaluation of its in vitro and in vivo properties. To this end, a L-proline-graft-poly(HEMA) was prepared and labeled with 99mTc. The radiochemical yield of approximately the 99mTc-labeled compound amounted to 97 ± 2.3%. The cytotoxicity test revealed no cytotoxic effect after a 24- and 48-h incubation. The results of the hemolysis test showed that hemolysis was non-toxic with an effect level of less than 2%. Subsequently, the biodistribution in healthy rats was determined. High accumulation of the polymer was observed in the pancreas, thyroid and prostate.