Effect of verapamil on responses to endothelin-1 in aortic rings from streptozotocin-induced diabetic rats

DURMUŞ SÜTPİDELER N., KALKAN Ş., GİDENER S.

Pharmacological Research, vol.40, no.1, pp.37-40, 1999 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 1
  • Publication Date: 1999
  • Doi Number: 10.1006/phrs.1998.0485
  • Journal Name: Pharmacological Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.37-40
  • Keywords: Aorta, Diabetes mellitus, Endothelin-1, Rat, Verapamil
  • Manisa Celal Bayar University Affiliated: No

Abstract

In this study, we investigated the constrictor responsiveness to endothelin-1 (ET-1, 10-30 nM) of aortic rings (under 1 g resting tension in Krebs-Bicarbonate solution) from 8-weeks streptozotocin (STZ, 65 mg kg-1, i.p)-induced diabetic rats and vehicle-treated control rats. The maximum ET-1-induced contraction of the aorta in diabetic rats was increased by 150%, but the EC50 of ET-1 remained unchanged. Although in both groups, verapamil reduced the constrictor responses to ET-1 (diabetic group P < 0.001, control group P < 0.05), there were not any significant differences between PD2 values. These results suggest that verapamil inhibits ET-1-induced Ca2+ entry through the L-type channel and this effect did not change in diabetes mellitus.