Investigation of poly-β-hydroxybutyrate (PHB) production by Bacillus subtilis ATCC 6633 Under different conditions Farklı koşullar altında Bacillus subtilis ATCC 6633 tarafından poli-β-hidroksibütirat (PHB) üretiminin araştırılması

Tamdoǧan N., Sidal U.

Kafkas Universitesi Veteriner Fakultesi Dergisi, cilt.17, sa.SUPPL. A, 2011 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: SUPPL. A
  • Basım Tarihi: 2011
  • Dergi Adı: Kafkas Universitesi Veteriner Fakultesi Dergisi
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Anahtar Kelimeler: Bacillus subtilis, Bioplastic production, Carbon and nitrogen sources, pH, Poly-β-hydroxybutyric asid (PHB), Temperature
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

The aim of our study was to assess the PHB productivity of Bacillus subtilis ATCC 6633 strain under different conditions. PHB amount was measured by UV-VIS spectrophotometer at 235 nm. The effects of the incubation time, temperature, pH, carbon sources, nitrogen sources and the rate of carbon/nitrogen ratio on PHB production rate were investigated. The results demonstrated that the highest PHB production was obtained after 24 h of incubation (10.4981 μg/ml), at 7.0 of pH (10.4981 μg/ml), with D-mannitole (23.6623 μg/ml) as carbon source, L-glycine (14.6217 μg/ml) as nitrogen source, C/N ratio of 2.5 (3.2481 μg/ml) and with a temperature of 30°C in culture media (10.4981 μg/ml).