Augmentation of methylprednisolone-induced differentiation of myeloid leukemia cells by serine/threonine protein phosphatase inhibitors

Uzunoglu S., Uslu R., TÖBÜ M., Saydam G., Terzioglu E., Buyukkececi F., ...More

Leukemia Research, vol.23, no.5, pp.507-512, 1999 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 5
  • Publication Date: 1999
  • Doi Number: 10.1016/s0145-2126(99)00040-5
  • Journal Name: Leukemia Research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.507-512
  • Keywords: leukemia, differentiation, methylprednisolone, phosphatase inhibitors, okadaic acid, calyculin A
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

To elucidate the roles of serine/threonine protein phosphatases type 1 (PP1) and type 2A (PP2A) in methylprednisolone-induced differentiation of HL60 cells into granulocytes and K562 cells into monocytes, we examined the effect of serine/threonine protein phosphatase inhibitors, okadaic acid and Cal-A on the proliferation/differentiation of HL60 and K562 cells. Okadaic acid and Cal-A augmented methylprednisolone induced granulocytic differentiation and cell death of HL60 cells and monocytic differentiation and cell death of K562 cells in different dose ranges, respectively. These data suggest an important role of PP1 and PP2A in the mechanism leading to differentiation of leukemic cells.