Cultivation of Leishmania sp. in nutrient broth.

ÖZBİLGİN A., ÖZBEL Y., Alkan M., Atambay M., Ozcel M.

Journal of the Egyptian Society of Parasitology, vol.25, no.2, pp.437-441, 1995 (Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 25 Issue: 2
  • Publication Date: 1995
  • Journal Name: Journal of the Egyptian Society of Parasitology
  • Journal Indexes: Scopus
  • Page Numbers: pp.437-441
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

Bone-marrow aspiration and biopsy material samples obtained from two patients, one diagnosed as visceral and other as cutaneous leishmaniasis, were inoculated in Novy, McNeal, Nicolle (NNN) medium and nutrient broth (NB), containing fetal calf serum (FCS), penicillin and streptomycine. Both media were incubated at 27 degrees C for 10 days and observed daily for L. infantum and L. major promastigotes. Promastigotes were observed in nutrient broth after the first day, while in NNN media after the second or third day of incubation, indicating the effectiveness of nutrient broth in early diagnosis of both forms of leishmaniasis.