A new experimental in vitro culture medium for cultivation of Leishmania species

LİMONCU M. E., BALCIOĞLU İ. C., YERELİ K., ÖZBEL Y., ÖZBİLGİN A.

Journal of Clinical Microbiology, vol.35, no.9, pp.2430-2431, 1997 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 35 Issue: 9
  • Publication Date: 1997
  • Doi Number: 10.1128/jcm.35.9.2430-2431.1997
  • Journal Name: Journal of Clinical Microbiology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2430-2431
  • Manisa Celal Bayar University Affiliated: Yes

Abstract

A new liquid culture medium prepared with chemicals that can be obtained economically and commercially was tested in in vitro cultivation of Leishmania promastigotes to obtain a large number of organisms to use in serological studies. The number of Leishmania infantum and Leishmania tropica promastigotes taken from Novy-MacNeal-Nicolle (NNN) medium reached 1 x 107/ml at the end of the 8th day in our new medium, though in NNN medium the number of organisms reached only 5 x 106/ml. After 10 subsequent passages, the culture medium prepared was evaluated as being quite inexpensive, simple, and successful compared with other commercially available liquid culture media.