A new experimental in vitro culture medium for cultivation of Leishmania species

LİMONCU M. E., BALCIOĞLU İ. C., YERELİ K., ÖZBEL Y., ÖZBİLGİN A.

Journal of Clinical Microbiology, cilt.35, sa.9, ss.2430-2431, 1997 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 9
  • Basım Tarihi: 1997
  • Doi Numarası: 10.1128/jcm.35.9.2430-2431.1997
  • Dergi Adı: Journal of Clinical Microbiology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2430-2431
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

A new liquid culture medium prepared with chemicals that can be obtained economically and commercially was tested in in vitro cultivation of Leishmania promastigotes to obtain a large number of organisms to use in serological studies. The number of Leishmania infantum and Leishmania tropica promastigotes taken from Novy-MacNeal-Nicolle (NNN) medium reached 1 x 107/ml at the end of the 8th day in our new medium, though in NNN medium the number of organisms reached only 5 x 106/ml. After 10 subsequent passages, the culture medium prepared was evaluated as being quite inexpensive, simple, and successful compared with other commercially available liquid culture media.