One-step conjugation of glycylglycine with [18F]FDG and a pilot PET imaging study

Şenk A., İÇHEDEF Ç., Kılçar A., Uçar E., Arı K., Göksoy D., ...Daha Fazla

Journal of Radioanalytical and Nuclear Chemistry, cilt.316, sa.2, ss.457-463, 2018 (SCI-Expanded, Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 316 Sayı: 2
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s10967-018-5772-x
  • Dergi Adı: Journal of Radioanalytical and Nuclear Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.457-463
  • Anahtar Kelimeler: FDG, Glycylglycine, Imaging, PET, Radiopharmaceuticals
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

This study describes a single step conjugation of Glycylglycine (GlyGly) which is a small peptide, with [18F]FDG via oxime formation. Amiooxy-functionalization of GlyGly (AO-GlyGly) was accomplished through the reaction of Boc-aminooxy succinimide ester. Conjugation reaction was performed at 100 °C for 30 min in a vial containing AO-GlyGly and [18F]FDG solution. The radiolabeled product ([18F]FDG-GlyGly) was obtained with 98.65 ± 0.35% yield without any purification step which makes this method more attractive for 18F radiolabeling. The present study is concluded with an in vivo pilot animal PET study to assess biodistribution and kinetics of chemoselectively [18F]FDG tagged GlyGly in vivo.