Biosensing approach for glutathione detection using glutathione reductase and sulfhydryl oxidase bienzymatic system

TİMUR S., ODACI DEMİRKOL D., DİNÇER A., ZİHNİOĞLU F., Telefoncu A.

TALANTA, sa.5, ss.1492-1497, 2008 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.talanta.2007.09.026
  • Dergi Adı: TALANTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1492-1497
  • Anahtar Kelimeler: glutathione reductase, sulfhydryl oxidase, reduced/oxidized glutathione, electrochemical detection
  • Manisa Celal Bayar Üniversitesi Adresli: Hayır

Özet

Chitosan membrane with glutathione reductase and sulfhydryl oxidase (SOX) was subsequently integrated onto the surface of spectrographic graphite rods for obtaining a glutathione biosensor. The working principle was based on the monitoring of O-2 consumption that correlates the concentration of glutathione during the enzymatic reaction. A linear relationship between sensor response and concentration was obtained between 0.5 and 2.0 mM for oxidized glutathione (GSSG), and 0.2-1.0 mM for reduced glutathione (GSH) in the presence of 2 mu M nicotinamide adenine dinucleotide phosphate (NADPH) under the optimum working conditions. Also, reduced/oxidized glutathione were separated by HPLC and utility of bienzymatic system was investigated as an electrochemical detector for the analysis of these compounds. All data were given as a comparison of two systems: biosensor and diode array detector (DAD). (C) 2007 Elsevier B.V. All rights reserved.