Immobilization of α-glucosidase in chitosan coated polygalacturonic acid

DİNÇER A., OKUTUCU B., ZİHNİOĞLU F., Telefoncu A.

PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, sa.2, ss.103-111, 2005 (SCI-Expanded, Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2005
  • Doi Numarası: 10.1081/pb-200054695
  • Dergi Adı: PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.103-111
  • Anahtar Kelimeler: alpha-glucosidase, chitosan coated polygalacturonic acid (PGA) beads, immobilization
  • Manisa Celal Bayar Üniversitesi Adresli: Hayır

Özet

Crude alpha-glucosidase from Baker's yeast was immobilized in polygalacturonic acid beads and coated with chitosan. Chemical and physical characterization were performed by using p-nitrophenyl-alpha-D-glucopyranoside (pNPG) as an artificial substrate. Operation, thermal, pH, and strorage stabilities of the free and immobilized enzyme were also examined. The stabilities of immobilized enzyme were found to be better than that of the free enzyme. Furthermore, the hydrolysis rate of the chitosan coated alpha-glucosidase polygalacturonic acid beads were studied. In conclusion, the enzyme beads appear to have good characteristics and offer the prospect that this system may find application in enzyme immobilization, in addition to controlled drug release studies.