Akademik Veri Yönetim Sistemi
Turk Uroloji Dergisi, cilt.31, sa.1, ss.17-20, 2005 (Scopus)
Introduction: Dimethyl Sulphoxide (DMSO), with exceptional solvent properties, is used in various pharmacological studies on corpus cavernosum as a vehicle. However, there is little known regarding the relaxing characteristics of this solvent. Thus we aimed to determine the highest noneffecious concentration of DMSO as a solvent in isolated human corpus cavernosum (HCC). Materials and methods: Strips (3×3×7 mm) were cut longitudinally from human corpus cavernosum (HCC) tissues and mounted in 10 ml organ bath with the Krebs solution. The strips were oxygenated with 95% O2 and 5% CO2 and the temperature was maintained at 37°C. HCC tissues were contracted with 3×10-6 M phenylephrine and relaxation responses were evaluated by cumulative (10-9 - 10-5 M) and (10-12 - 10-9 M) addition of DMSO. Results: Cumulative application of phenylephrine (10-8 - 10-5 M) caused concentration dependent contraction response in HCC tissue (pD2 6.24±0.14). DMSO (10-9 - 10-5M) had dose dependent relaxing effects on precontracted HCC tissues with phenylephrine (3×10-6 M). HCC tissues exhibited relaxation response to DMSO 29±5.4% of phenylephrine-induced tone (IC50 6.63±0.4). At lower concentration (10-12 - 10-9 M) DMSO did not cause relaxation response curve. Conclusion: In this study we demonstrated that DMSO could be used in isolated HCC studies as a solvent at concentrations lower than 10-9 M.