Evaluation of the performance of maldi-tof ms and dna sequence analysis in the identification of mycobacteria species

Akyar I., Çavuşoğlu C., AYAŞ M., SÜRÜCÜOĞLU S., İlki A., Kaya D. E., ...Daha Fazla

Turkish Journal of Medical Sciences, cilt.48, sa.6, ss.1351-1357, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 48 Sayı: 6
  • Basım Tarihi: 2018
  • Doi Numarası: 10.3906/sag-1801-198
  • Dergi Adı: Turkish Journal of Medical Sciences
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.1351-1357
  • Anahtar Kelimeler: Identification, MALDI-TOF MS, Mycobacterium, Mycobacterium tuberculosis complex, Nontuberculous mycobacteria
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

Background/aim: Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is an alternative way of identifying mycobacteria via the analysis of biomolecules. It is being increasingly used in routine microbiology practice since it permits early, rapid, and cost-effective identification of pathogens of clinical importance. In this study, we aimed to evaluate the efficacy of phenotypic identification of mycobacteria by the MALDI-TOF MS MBT Mycobacteria Library (ML) 4.0 (Bruker, Daltonics) compared to standard sequence analysis. Materials and methods: A total of 155 Mycobacterium clinical and external quality control isolates, comprising nontuberculous mycobacteria (NTM) (n = 95) and the Mycobacterium tuberculosis complex (MTC) (n = 60), were included in the study. Results: Identification by MBT ML4.0 was correctly performed in 100% of MTC and in 91% of NTM isolates. All of the MTC isolates were correctly differentiated from NTM isolates. Conclusion: Based on our results, MBT ML4.0 may be used reliably to identify both NTM and MTC.