Evaluation of 131I-Pentamidine for scintigraphy of experimentally Leishmania tropica-infected hamsters

İNCEBOZ T., Lambrecht F. Y., Eren M. Ş., GİRGİNKARDEŞLER N., BEKİŞ R., YILMAZ O., ...Daha Fazla

Journal of Drug Targeting, cilt.22, sa.5, ss.416-420, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 22 Sayı: 5
  • Basım Tarihi: 2014
  • Doi Numarası: 10.3109/1061186x.2013.878943
  • Dergi Adı: Journal of Drug Targeting
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.416-420
  • Anahtar Kelimeler: Amastigote, I-131-Pentamidine scintigraphy, Leishmania tropica, Leishmaniasis hamster, promastigote
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

We aimed to assess the ability of 131I-Pentamidine scintigraphy to detect the lesions of Leishmania tropica infection. An experimental model of cutaneous leishmaniasis was developed. The presence of cutaneous leishmaniasis was confirmed. Pentamidine was radioiodinated with 131I. The radiolabeled pentamidine was validated by the requisite quality control tests to check its radiolabeling efficiency, in vitro stability. 131I- Pentamidine (activity: 18.5 MBq/100 l) was injected intracardiacally into infected hamsters. Static whole body images of the hamsters were acquired under the gamma camera at 5 and 30 in, 2, 6 and 24 h following the administration. On the scintigrams, anatomically adjusted regions of interest (ROIs) were drawn over the right feet (target) and left feet (not-target) and various organs. Accumulation of 131I-Pentamidine at sites of infection is expressed as the target to non-target (T/NT) ratio. The results T/NT ratio decreased with time. In concluding the 131I-Pentamidine has poor sensitivity in detection of L. tropica infection. © 2014 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted.