Akademik Veri Yönetim Sistemi
Turkish Journal of Biochemistry, cilt.40, sa.6, ss.481-491, 2015 (SCI-Expanded)
Objective: Physical process parameters play a major role in the cultivation of cyanobacteria to provide high yield. The aim of this study was to optimize physical parameters such as light intensity and agitation rate which might affect the phycobiliprotein formations for cyanobacterial strains of Oscillatoria agardhii and Synechococcus nidulans using response surface methodology. Methods: The cyanobacterial strains were cultured in 250 mL flasks containing 100 mL of EM medium in orbital shaking incubator under the temperature of 22±2°C at different light intensities and agitation rates for 10 days. The experimental design was carried out using 22 full-factorial experiments design with four axial points (α=1.414) and five replicates at the central point (65 μmol photons m-2s-1 and 150 rpm), according to the central composite design. Results: The optimization solution of O. agardhii (approximately at 156 rpm under the light intensity of 65 μmol photons m-2s-1) was selected because it resulted in the highest predicted response with the highest desirability. Furthermore, the optimization solution for S. nidulans suggested the phycobiliprotein amount of 9.95 mg/L obtaining at the agitation rate of 185 rpm under the light intensity of 46 μmol photons m-2s-1. The optimized results were reliable and the regions studied were proven to be statistically adequate. Conclusion: High agitation rate stimulated the faster growth than increased the light intensity for the growths of cyanobacterial strains.