Effect of geldanamycin on the expression of the matrix molecules and angiogenetic factors in a gastric cancer cell line

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Gürpınar T., KOSOVA F., Kurt F., Cambaz S., Yücel A., UMUR N., ...More

Biotechnic and Histochemistry, vol.96, no.2, pp.111-116, 2021 (SCI-Expanded, Scopus)

• Publication Type: Article / Article
• Volume: 96 Issue: 2
• Publication Date: 2021
• Doi Number: 10.1080/10520295.2020.1772507
• Journal Name: Biotechnic and Histochemistry
• Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
• Page Numbers: pp.111-116
• Keywords: 17-allylamino-17-demethoxygeldanamycin, angiogenesis, cell matrix, endostatin, gastric cancer, geldanamycin, metalloproteinases, thrombospondin, vascular endothelial growth factor
• Manisa Celal Bayar University Affiliated: Yes

Abstract

Angiogenesis is the formation of new blood vessels. Angiogenesis affects cancer growth and is a useful target for cancer therapeutics. The effects of geldanamycin on angiogenesis in cases of gastric cancer are poorly understood. We investigated the effects of different doses of 17-allylamino-17-demethoxygeldanamycin (17-AGG), a semi-synthetic derivative of geldanamycin, on the interactions between cellular matrix proteins and angiogenesis factors in a gastric cancer cell line. We examined cancer cells on laminin and collagen I coated surfaces to determine their response to the angiogenic effect of these matrix molecules. We also evaluated the expression levels of VEGF, MMP-9, ES and TSP-1 using ELISA. We found that application of 17-AAG to the gastric cancer cell line on culture dish plastic decreased VEGF, TSP-1, ES and MMP-9 expression, whereas of all of these proteins were increased by laminin and collagen coating. 17-AAG currently is in clinical trial phase 2 and may be a promising drug for treatment of gastric cancer.