Effect of geldanamycin on the expression of the matrix molecules and angiogenetic factors in a gastric cancer cell line

Gürpınar T., KOSOVA F., Kurt F., Cambaz S., Yücel A., UMUR N., ...Daha Fazla

Biotechnic and Histochemistry, cilt.96, sa.2, ss.111-116, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 96 Sayı: 2
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1080/10520295.2020.1772507
  • Dergi Adı: Biotechnic and Histochemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.111-116
  • Anahtar Kelimeler: 17-allylamino-17-demethoxygeldanamycin, angiogenesis, cell matrix, endostatin, gastric cancer, geldanamycin, metalloproteinases, thrombospondin, vascular endothelial growth factor
  • Manisa Celal Bayar Üniversitesi Adresli: Evet

Özet

Angiogenesis is the formation of new blood vessels. Angiogenesis affects cancer growth and is a useful target for cancer therapeutics. The effects of geldanamycin on angiogenesis in cases of gastric cancer are poorly understood. We investigated the effects of different doses of 17-allylamino-17-demethoxygeldanamycin (17-AGG), a semi-synthetic derivative of geldanamycin, on the interactions between cellular matrix proteins and angiogenesis factors in a gastric cancer cell line. We examined cancer cells on laminin and collagen I coated surfaces to determine their response to the angiogenic effect of these matrix molecules. We also evaluated the expression levels of VEGF, MMP-9, ES and TSP-1 using ELISA. We found that application of 17-AAG to the gastric cancer cell line on culture dish plastic decreased VEGF, TSP-1, ES and MMP-9 expression, whereas of all of these proteins were increased by laminin and collagen coating. 17-AAG currently is in clinical trial phase 2 and may be a promising drug for treatment of gastric cancer.